Auxins and Cytokinins Involved in Micropropagation of Pepino Plant (Solanum muricatumAiton)

Waad S Faizy,Yousif S Tamer,Wisam Khaza'Al,Rafail S Toma

doi:10.52951/dasj.21130103

Abstract

A reliable and successful micropropagation protocol was developed for pepino plant (Solanum muricatum Aiton) from nodal segment explants grown on MS medium. The best values of shoot multiplication traits were recorded from the addition of 3 mg.l-1 kinetin by producing 2.3 shoots/explant, 3.6 cm and 9.6 leaves/ explant which was significantly superior upon the addition of BA at the same levels. In case of adding 3 mg.l-1 kinetin, the best root formation attributes were achieved from the use of 0.2 mg.l-l IAA that resulted a maximum number of roots (14.33 roots/ explant). The longest root length (15.33 cm) was achieved when 0.3 mg.l-l IAA was used. A 100% rooting percentage was recorded from the all tested auxins including IAA, IBA and NAA. The gradually moved plantlets from the heterotrophic phase in the lab to the autotrophic phase in the greenhouse showed 100% success. The plantlets did not show any abnormal growth or morphological changes. It is concluded that this important plant can be easily propagated by tissue culture technique through a reliable micropropagation protocol.

Highlights

Pepino dulce is the common name of Solanum muricatum, it belongs to the family solanaceae and genus Solanum (Ipgri and Comav, 2004)
A reliable and successful micropropagation protocol was developed for pepino plant (Solanum muricatum Aiton) from nodal segment explants grown on MS medium
Amost 100% of healthy cultures were achieved from the initiation stage of pepino nodal segment explants grown on MS medium after a successful disinfestation with NaOCl (10%) for 10 minutes (Fig.1, A)

Summary

Introduction

Pepino dulce is the common name of Solanum muricatum, it belongs to the family solanaceae and genus Solanum (Ipgri and Comav, 2004). It is one of the subtropical evergreen shrubs natively present in several countries of South America. It is commercially propagated in Australia, USA, New Zealand, Turkey, etc. It comes in different colors and shapes. The name pepino comes from the Spanish language which means“cucumber” because it is similar to cucumber in texture and taste especially at the premature stage but when it matures, the taste becomes sweet that is why it is called sweet cucumber (Orhanet al., 2014).

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Results