Auxin herbicides, halosulfuron, sulfentrazone, and topramezone disparately affect morphology and ultraviolet features of weedy flowers and associated pollinator foraging

Abstract

Abstract Pollinators risk exposure to insecticide residue when visiting weedy flowers in urban landscapes. Previous research shows that pollinators are routinely exposed to a variety of pesticides, but herbicides have exhibited minimal toxicity and did not contribute to the modeled risk quotients. Herbicides from different modes of action may deter pollinators from visiting turfgrass weeds, but their temporal influence on floral quality and pollinator foraging is unaddressed. Research experiments were conducted at Blacksburg, VA, in 2023 to assess the effect of four herbicides on floral morphology and ultraviolet (UV) reflectance of three different UV floral classes of weeds and associated pollinator foraging visits. Among 1,080 assessments per weed species, honeybees (Apis mellifera), bumble bees (Bombus spp.), solitary bees (Chelostoma florisomne), and flies (Diptera spp.) accounted for 94%, 2%, 3%, and 1%, respectively, of the total pollinator visitations on white clover (Trifolium repens L.) inflorescences; 71%, 2%, 0%, and 27%, respectively, on dandelion (Taraxacum officinale F.H. Wigg.) flowers; and 0%, 0%, 78%, and 22%, respectively, on bulbous buttercup (Ranunculus bulbosus L.) flowers. Pollinator visitation and floral quality were temporarily affected by herbicide application, with some herbicides eliminating food resources, while others transiently impacted floral quality and density. The combination of 2,4-D + dicamba + MCPP and topramezone eliminated pollinator foraging visits, but on differing temporal scales of 3 d for auxins and 14 d for topramezone. Halosulfuron and sulfentrazone transiently suppressed floral quality and density, with varying degrees of deterrence on pollinators depending on the weed species. All evaluated herbicides reduced radiometric UV reflectance of T. officinale petal apices, but only synthetic auxin and topramezone reduced digitally assessed floral UV-reflecting area. Petal UV reflectance appears to contribute but not solely influence pollinator foraging behavior. UV-absorbing and UV-reflecting flowers differed in UV-reflectance response to herbicides, but pollinators were similarly deterred. Results suggest that herbicides may offer a variety of management solutions to pollinator deterrence in areas slated for insecticide treatment, including long-term or transient deterrence with potential food-resource preservation.