Auxin, Cytokinin, and Ethylene Involved in Rice N Availability Improvement Caused by Endophyte Phomopsis liquidambari

Abstract

Nitrogen (N) is one of the most limiting nutrients for rice yield. There is mounting evidence that the endophytic fungus Phomopsis liquidambari B3 can establish a mutualistic symbiotic relationship with rice, enhancing N uptake and metabolism in rice (Oryza sativa L.). To examine the mechanism underlying the effect of B3 on nitrogen accumulation and metabolism in rice plants, a pot experiment was conducted to examine the N and phytohormone levels in response to endophyte infection at four whole growth durations during exposure to different N levels. Our results showed that the contents of auxin, cytokinin, and ethylene in rice were significantly enhanced by B3 under low N levels at different growth durations; B3 symbiosis increased N accumulation and rice yield and induced the expression of some genes related to N uptake and metabolism. To further verify that B3 symbiosis enhances N use in rice by regulating phytohormones, we performed a hydroponic experiment in which exogenous phytohormones and their specific inhibitors were applied. The results showed that the application of exogenous auxin, cytokinin, and ethylene increased the rice content of nitrogen, and their inhibitors decreased the amount of nitrogen absorbed in rice. As expected, B3 infection alleviated the negative effect caused by inhibitors slightly. In summary, we conclude that P. liquidambari symbiosis may regulate the content of auxin, cytokinin, and ethylene to improve N use in rice.