Abstract
Nowadays, natural microbial pigments are emerging as potential candidate therapeutic agents that can be used in antibiotic resistance. In this paper, we report the antimicrobial activity of green algae pigments. Green algae SC3 isolate was isolated from soil in Erzurum. Pigments extracted by methanol: dimethyl sulfoxide solvents were partially characterized by Thin Layer Chromatography (TLC), UV-Absorbance, and Fourier-transform infrared spectroscopy (FTIR). The antimicrobial activity of pigments was determined with agar well diffusion and microbroth assays against Pseudomonas aeruginosa (ATCC 27853 and clinic isolate), Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 25922). To identify green algae, total DNA was isolated and both 18S rRNA and 16S rRNA for the chloroplast genome were amplified using universal primers. Thus, this alga was identified as Auxenochlorella protothecoides and submitted to Genbank (Accession number: MW139225 and MW063613). According to our results, the best antimicrobial activity was recorded against P. aeruginosa. Our results show for the first time the antimicrobial activity of the total pigments from A. protothecoides green algae.
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