Abstract
Effects of a suspected toxin in isolated microspore cultures of oilseed rape, Brassica napus L., were observed in the reduced levels of embryogenesis and in altered embryo morphology. The toxin appeared to be generated by the cultured spores themselves. Inclusion of binucleate microspores in the cultures, along with younger, potentially embryogenic spores, was correlated with these negative effects and could be simulated by inoculation of embryogenic cultures with media generated from high-density cultures of older spores. Replacement of culture media 24 h after microspore isolation effectively reduced the levels of the autotoxins in the medium and allowed normal embryo development.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
