Abstract
Allotype-suppressed rabbits were injected with immunizing material containing the ‘suppressed’ allotypic specificity (Ab9). This invariably resulted in formation of antibodies against Ab9, even if the immunized animals already started to recover from suppression and had circulating Ab9 in concentrations as high as 1% of the normal homozygous value. By various techniques it was possible to detect Ab9 and anti-Ab9 present simultaneously in sera of a large proportion of animals. Antisera raised in suppressed-immunized rabbits were found to contain antibodies directed only to some determinants of the Ab9 allotype. Circulating antibodies did not react with autologous antigen, but reacted with antigens from sera of other suppressed-immunized rabbits. At least some of the antisera contained also autoantibodies which reacted with autologous antigens. These ‘hidden’ autoantibodies were isolated by Sephadex G-200 chromatography; they reacted with Ab9 antigen isolated from the same serum sample.
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