Abstract
Many regions around the third ventricle of the brain are thought to participate in the regulation of water intake. In this study we have visualized in acutely hyperosmolar mice the hypothalamic regions by using autoradiography and [14C]deoxyglucose as marker. By applying a recently published, inexpensive, calibrated photographic method for the analysis of the autoradiographs, we can show that the medial parts of the hypothalamus near the third ventricle increased their uptake of deoxyglucose in comparison with the lateral parts. Densitometric measurements confirmed the results. The results also indicate that parts of the medial hypothalamus, even those that are more caudal than the paraventricular nuclei, react to hyperosmolarity.
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