Autoradiographic localization of muscarinic acetylcholine receptors in the rat pulmonary vascular tree

Abstract

The pharmacological characteristics and the anatomical localization of muscarinic receptors in the pulmonary vascular tree were investigated in lung sections of Wister-Kyoto (WKY) and spontaneously hypertensive rats (SHR). [ 3H]Quinuclidinyl benzylate ([ 3H]QNB) was bound by sections of rat lung in a manner consistent with the labeling of muscarinic acetylcholine receptors, with a dissociation constant value (K d) of 0.41 ± 0.3 nM in WKY rats and of 0.37 ± 0.2 nM in SHR. The density of muscarinic acetylcholine receptors was higher in sections of lung of WKY rats than of SHR. In the pulmonary vasculature these sites were associated with the smooth muscle of the medial layer of different size branches of the pulmonary artery and vein. No [ 3H]QNB binding sites were found within the endothelium in the blood vessels of either WKY rats or SHR. The density of [ 3H]QNB binding sites was significantly lower in the smooth muscle of pulmonary vein and its branches in SHR. There were no significant hypertension-dependent changes in the density and pattern of muscarinic receptors of pulmonary artery smooth muscle.