Abstract
Many of the characteristics of [ 3H]desipramine ([ 3H]DMI) binding sites, previously established with brain homogenates, were confirmed in slide-mounted sections of rat forebrain. A 1:1 ratio of specific to non-specific binding was established. The proport on of specifically bound [ 3H]DMI was reduced in brain sections from rats pretreated with 6-hydroxydopamine but not with 5,6-dihydroxytryptamine. High resolution localization of [ 3H]DMI binding sites was achieved by exposing the sections to tritium-sensitive film.
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