Autophagy-related gene expression is an independent prognostic indicator of glioma.

Huixue Zhang,Jianlong Li,Xueling Zhou,Jie Li,Yuze Cao,Yang Jiao,Yonghui Pan,Xiaoyan Lu,Ning Wang,He-Ping Ma,Xiaokun Wang,Lei Yang,Tianfeng Wang,Lihua Wang,Jianjian Wang,Shangwei Ning,Lin Cong

doi:10.18632/oncotarget.17719

Abstract

In this study, we identified 74 differentially expressed autophagy-related genes in glioma patients. Analysis using a Cox proportional hazard regression model showed that MAPK8IP1 and SH3GLB1, two autophagy-related genes, were associated with the prognostic signature for glioma. Glioma patients from the CGGA batches 1 and 2, GSE4412 and TCGA datasets could be divided into high- and low-risk groups with different survival times based on levels of MAPK8IP1 and SH3GLB1 expression. The autophagy-related signature was an independent predictor of survival outcomes in glioma patients. MAPK8IP1 overexpression and SH3GLB1 knockdown inhibited glioma cell proliferation, migration and invasion, and improved Temozolomide sensitivity. These findings suggest autophagy-related genes like MAPK8IP1 and SH3GLB1 could be potential therapeutic targets in glioma.

Highlights

Autophagy is a dynamic process that degrades intracellular constituents in double membrane-bound vesicles during stress or nutrient deprivation [1]
Recent studies have indicated that autophagy enables tumor cell survival or induces cell death depending on the cellular context [4]
We investigated the role of MAPK8IP1 and SH3GLB1 on glioma cell proliferation, migration, invasion and their effect on Temozolomide treatment

Summary

Introduction

Autophagy is a dynamic process that degrades intracellular constituents in double membrane-bound vesicles (autophagosomes) during stress or nutrient deprivation [1]. Recent studies have indicated that autophagy enables tumor cell survival or induces cell death depending on the cellular context [4]. Autophagy can suppress early stages of cancer development by eliminating damaged proteins and organelles, thereby mitigating cellular damage and limiting chromosomal instability [5, 6]. It can promote tumor growth in low oxygen and nutrient conditions [7, 8]. Recent studies indicate that inhibition of autophagy suppresses tumor growth, promotes tumor cell death and overcomes therapy resistance [9]. Prognostic biomarkers for glioma based on autophagy have not been identified

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Conclusion