Autophagy inhibitor Spautin-1 sensitizes osteosarcoma cells to cisplatin-induced apoptosis

Abstract

Objective To study the effect of autophagy inhibitor Spautin-1 on osteosarcoma cell sensitivity to cisplatin-induced cell proliferation inhibition and apoptosis. Methods Osteosarcoma cell line U2OS cells were treated with an effective inhibition concentration of Spautin- 1(10 μmol/L). Cell proliferation was measured by cell counting kit- 8(CCK- 8), and cell apoptosis was measured by flow cytometry and Western blotting. Results Spautin-1 has no obvious effect on U2OS cell proliferation and apoptosis(P> 0.05). However, Spautin-1 sensitized osteosarcoma cells to cisplatin-induced cell proliferation inhibition and apoptosis. The flow cytometry indicated that the apoptosis rate in Spautin-1+cisplatin group(35.0%) was significantly higher than that in cisplatin group(16.5%). The cleaved Caspase-3 and poly adenosine diphosphate-ribose polymerase (PARP) were also increased in Spautin-1+cisplating group as compared with cisplatin group. Conclusion The autophagy inhibitor Spautin-1 sensitizes osteosarcoma cells to cisplatin-induced cell proliferation inhibition and apoptosis. Key words: Spautin-1; Osteosarcoma; Proliferation; Chemosensitivity