Abstract
Objective To investigate the relationship between oncogenic H-RasV12 overexpression/activation and the autophagic activity by observing the effect of Ras overexpression on autophagic activity in human fibroblast cells.MethodsHuman BJ fibroblast cells were transfected with H-RasV12 or control vector,and then the cellular responses to H-RasV12 overexpression were analyzed by observing the morphology,cell growth curve,senescence-associatedβ-Gal staining,Western blotting analysis,flow cytometry,and suppression of autophagy-related protein 5(ATG5)by siRNA.Results Compared with control group,BJ cells overexpressing H-RasV12 developed prominent premature senescence and inhibited autophagic activity,as manifested by significant accumulation of p62 and light chain 3Ⅱ(LC3Ⅱ).The autophagy inhibition by H-RasV12 remained stable during the study period;the apoptosis rate was increased in H-RasV12 overexpressing BJ cells compared with that in the control cells.Suppression of ATG5 by siRNA led to more severe senescence in Ras-overexpressing BJ cells.Conclusion Our results suggest that the autophagy activity is inhibited in human fibroblast cells stably overexpressing oncogenic H-RasV12,and the inhibition is in the later stage of autophagy,which may be related to H-RasV12-related tumorigenesis.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Academic Journal of Second Military Medical University
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
