Abstract
An automatic system for optimizing the conditions for the chromatographic separation of protamines has been developed using Nelder and Mead's modified simplex method. It is proposed to use penalty functions in order to take into account nonrigid constraints on the parameters to be optimized. The optimum conditions for the separation of the protamines from sturgeon gonads have been determined. It has been established that the greatest influence on the separation of the protamines is exerted by the concentration of the ion-pair reagent — trifluoroacetic acid. The system described permits the optimization of any chromatographic separations and the determinations to be performed thanks to the application of penalty functions to the optimization parameter.
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