Abstract
A stopped-flow spectrophotometer was automated using a digital transient waveform recorder interfaced to a commercial 8-bit microcomputer. An assembly language program was used to acquire the kinetic data from the transient waveform recorder. A non-linear least-squares program was used to determine the rate constants from the stopped-flow kinetic data. The performance of the system was evaluated using electronic simulation and reaction rate data from the reaction of 2,6-dichlorophenolindophenol with ascorbic acid. The effect of varying the number of data samples on the calculated rate constant was investigated.
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