Abstract
An automatic stopped-flow method is proposed for the routine determination of microgram amounts of l-cysteine, based on its oxidation by 2,6-dichlorophenolindophenol (DPIP) in a weakly basic medium. The oxidation reaction is monitored by measuring the rate of the absorbance decrease at 615 nm, the wavelength of the maximum absorption for DPIP. Under optimal conditions, the calibration graph is linear over the range 2–32 μg/ml and the detection limit is 300 ng/ml. Most of the amino-acids tested have no effect on the rate of the oxidation reaction and are tolerated at high concentration levels. The sample throughput achieved, 80 samples per hr, allows the proposed method to be used for the routine determination of l-cysteine.
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