Automatic procedure for non-coplanar aberration compensation in lensless Fourier transform digital holography

Abstract

As the lensless Fourier transform digital holography is applied into the microscopic phase-contrast imaging on the live cells, the motion of the cells will lead to the non-coplanarity phenomena between the object and the reference source. This could result in the imaging aberration. An effective and robust autofocus procedure based on the phase distribution is presented in the paper. With the initial measurement of the distance between the reference source and the hologram, the optimum parameters corresponding to the phase-contrast image can be achieved by a single hologram, combined with the linearity fitting. The lensless Fourier transform digital holographic system is built and the experiments on the phase-contrast imaging of the live cervical carcinoma cells are performed. Finally, the good experiment results are demonstrated. Both the theoretical analysis and the experimental investigation verify the feasibility and validity of the automatic procedure for the non-coplanar aberration compensation.