Automated synthesis and quality analysis of 18F-FMISO based on CFN-MPS-100 module

Abstract

Objective To synthesize 18F-FMISO and analyze the quality of the product. Methods 1-(2'-nitro-1'-imidazolyl)-2-O-tetrahydropyranyl-O-trluendulfonylpropanediol(NITTP) was taken as the precursor and simpleone potmethod was used. CFN-MPS-100 fluorine multifunction radiopharmaceutical chemical synthesis module was adopted to complete the radioactive fluorination reaction in a closed flat flask, and the crude product was purified by semi-preparative HPLC, the solvent was removed by rotary evaporation. Then 15 ml saline was added into the product to get 18F-FMISO injection. Radio-HPLC and radio-TLC were applied for quality control. Results 18F-FMISO was obtained in 60 min with the radiochemical yield of (32±5.0)% (no decay corrected, n=25). The radiochemical purity was above 99.0% and still above 98.5% after 6 h. The radioactive concentration was above 1.11×1012 Bq/L. The product was colorless solution, with pH value of 7.0. The radioactive nuclear purity was more than 99%. The K222 was less than 25 μg/ml. Conclusion 18F-FMISO could be synthesized with automatic synthesis method based on the CFN-MPS-100 fluorine multifunction module. The labeling rate, stability and chemical purity are high. Key words: FMISO; Isotope labeling; Fluorine radiotopes; Chemical synthesis