Abstract
AbstractA method for determining the isolated (nonconjugated)trans isomer content of fats and oils was developed using Fourier Transform Infrared Spectroscopy (FTIR), which permits automatic sample analysis and calculation of percenttrans content. Integrated band areas were used as a measure of the intensity of the band associated with thetrans C=C double bond. Measured band areas for samples with known percenttrans content were fit with a second order polynomial, resulting in a correlation coefficient of 0.99998 and a standard error of estimate of 0.11 over the range of 0 to 50%trans content. This technique also allows the analysis of neat samples, a significant improvement over current procedures in that it eliminates the need for dilution by volatile solvents and any attendant errors.
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