Abstract
Volta phase plate has shown great promise in single particle cryo-EM and cryo-Electron tomography due to the enhanced contrast introduced by the extra phase shift. It can resolve protein complexes less than 100KD to near-atomic resolution. But the lack of automation has hindered its wider adoption. Here we report a serialEM workflow that can automatically 1) change phase plate location, 2) condition the phase plate, and 3) perform astigmatism correction and coma-free alignment. We have used this workflow to collect a test dataset and achieve high-resolution structure determination.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
