Abstract
The separation of ten plant extracts using automated multiple development thin-layer chromatography (AMD-TLC) is described. Alcoholic extracts were obtained from Cinchona succirubra, Aesculus hippocastanum, Berberis vulgaris, Artemisia abrotanum, Carduus marianus, Thuja occidentalis, Baptisia tinctoria, Paulinia cupana, Lycopus europaeus and Echinacea angustifolia. The separation was performed on silica plates (Sil G-50 UV 254 (Macherey—Nagel), 10 × 20 cm). AMD was achieved in 25 steps using methanol, ethyl acetate, toluene, 1,2-dichloroethane, 25% ammonia solution and anhydrous formic acid as modifiers. The chromatograms were evaluated with a Shimadzu CS-9000 dual-wavelength flying-spot scanner. Better separations were obtained using AMD than isocratic elution.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
