Abstract
Recently, we developed simple vortex-mixing procedures for preparation of MLV, LUV, SUV and GUV, and succeeded in constructing “automated multi-functional liposome manufacturing equipment” adopting these novel procedures. This unique, easy-to-operate device possesses conventional vortex-mixing and ultrasonic parts and allows even unskilled users to prepare desired types of liposomes overnight with a single touch of a button. In addition to MLV, LUV, SUV and GUV, various functional liposomes, such as charged liposomes, sensitized liposomes, surfacemodified liposomes, and reconstituted liposomes, can be prepared. Comprising conventional parts, the fully automated machine operates under oxygen-/germ-free conditions and is also applicable for concentration of a solute in a solution by removal of organic solvent, biochemical reactions (as bioreactors), ultrasonic disruption of bacteria, etc.
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