Abstract
Methods for the measurement of 5-hydroxyindole-acetic acid (HIAA) in urine by highperformance liquid chromatography (HPLC) require a pre-extraction stage, which has not been automated. As well as automated sampling, the Gilson ASTED XL system (Anachem, Luton, Beds, UK) provides online extraction and concentration utilizing a trace enrichment cartridge (TEC) packed with an appropriate extractant. Sample preparation columns packed with an anion exchange resin, Isolute SAX (Jones Chromatography Ltd, Hengoed, MidGlamorgan, Wales), have been shown to be useful for the manual pre-extraction of urine for
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