Abstract
Aim of this study was to automate sampling and quantification of the previously described apparatus for combined determination of dissolution and permeation through Caco-2 monolayer by means of sequential injection analysis (SIA). Native fluorescence of propranolol HCl in Krebs–Ringer buffer (KRB) was used for quantification. Sampling was done at three different locations within the apparatus at a high sampling frequency (approximately 60 h −1). Injection volume delivered to the fluorescence detector was 50 μL for permeation monitoring and 25 μL for dissolution monitoring. Linear regression for 50 μL injection yielded a detection limit calculated as 0.04 μg mL −1 of propranolol HCl in KRB ( R 2 > 0.999). However, linearity for dissolution monitoring was not given for the complete range of concentrations and first order polynomial calibration was established ( R 2 > 0.9999). To conclude, the SIA system was able to monitor simultaneously dissolution and permeation of the immediate release propranolol HCl tablets and the authors succeeded in automating the apparatus for combined measurement of dissolution and permeation. In addition, the obtained data was consistent with data obtained by manual sampling followed by HPLC analysis.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
