Automated Measurement of Ovary Development in Atlantic Salmon Using Deep Learning

Abstract

Salmon breeding companies control the egg stripping period through environmental change, which triggers the need to identify the state of maturation. Ultrasound imaging of the salmon ovary is a proven non-invasive tool for this purpose; however, the process is laborious, and the interpretation of the ultrasound scans is subjective. Real-time ultrasound image segmentation of Atlantic salmon ovary provides an opportunity to overcome these limitations. However, several application challenges need to be addressed to achieve this goal. These challenges include the potential for false-positive and false-negative predictions, accurate prediction of attenuated lower ovary parts and resolution of inconsistencies in predicted ovary shape. We describe an approach designed to tackle these obstacles by employing targeted pre-training of a modified U-Net, capable of performing both segmentation and classification. In addition, a variational autoencoder (VAE) and generative adversarial network (GAN) were incorporated to rectify shape inconsistencies in the segmentation output. To train the proposed model, a data set of Atlantic salmon ovaries throughout two maturation periods was recorded. We then tested our model and compared its performance with that of conventional and novel U-Nets. The method was also tested in a salmon on-site ultrasound examination setting. The results of our application indicate that our method is able to efficiently segment salmon ovary with an average Dice score of 0.885 per individual in real-time. These results represent a competitive performance for this specific application, which enables us to design an automated system for smart monitoring of maturation state in Atlantic salmon.