Automated Integration of a UPLC Glycomic Profile.

Abstract

Ultra-performance liquid chromatography (UPLC) is the established technology for accurate analysis of IgG Fc N-glycosylation due to its superior sensitivity, resolution, speed, and its capability to provide branch-specific information of glycan species. Correct and cost-efficient preprocessing of chromatographic data is the major prerequisite for subsequent analyses ranging from inference of structural isomers to biomarker discovery and prediction of humoral immune response from characterized changes in glycosylation. The complexity of glycomic chromatograms poses a number of challenges for developing automated data annotation and quantitation algorithms, which frequently necessitated manual or semi-manual approaches to preprocessing, most notably to peak detection and integration. Such procedures are meticulous and time-consuming, and may be a source of confounding due to their dependence on human labelers. Although liquid chromatography is a mature field and a number of methods have been developed for automatic peak detection outside the area of glycomics analysis, we found that hardly any of them are suitable for automatic integration of UPLC glycomic profiles without substantial modifications. In this chapter, we illustrate practical challenges of automatic peak detection of UPLC glycomics chromatograms. We outline a robust, semi-supervised method ACE (Automatic Chromatogram Extraction) for automated alignment and detection of glycan peaks in chromatograms, developed by Pharmatics Limited (UK) in collaboration with Genos Limited (Croatia). Application of the tool requires minimal human interference, which results in a significant reduction in the time and cost of IgG glycomics signal integration using Waters Acquity UPLC instrument (Milford, MA, USA) in several human cohorts with blind technical replicas.