Abstract
Peroxisomes are known to proliferate under the influence of thyroid hormones. Using our automated image analysis system for light microscopy we investigated peroxisomal changes in number, size and staining intensity in hepatocytes from thyroxine (T4) and 3,3′,5′-triiodothyronine (T3) treated rats. The peroxisomes were specifically stained by the 3,3′-diaminobenzidine (DAB) reaction for catalase. The automated measurements are in agreement with the proliferation described in the literature, e.g. an increase in number and reduction in size, but the changes are less pronounced. The catalase reaction of the peroxisomes is decreased after treatment. The influence of different staining intensity levels upon the automatic delineation and morphometric data has been investigated. Volume and numerical densities are compared with data from other authors. Possible explanations for the discrepancies between our results and those of others are discussed.
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