Abstract
Automated hot solvent extraction of atrazine (ATR), desethylatrazine (DEA), and desisopropylatrazine (DIA) from soil samples has been optimized and variation of recoveries with total organic carbon content (TOC) in soil has been studied. Soil (20 g) was extracted for 30 min at an enhanced temperature with 120 mL of dichlormethane/acetone 65:35 v/v and, subsequently, rinsed 10 min with condensate. Extract was cleaned-up by gel permeation chromatography and an aliquot of triazine fraction was injected onto a narrow bore HPLC column packed with polymeric octadecyl phase. Mobile phase containing acetonitrile and 0.002 mol/L phosphate buffer of pH 6.8 was used for gradient elution. Recoveries higher than 98%, 93%, and 81% can be attained for ATR, DEA, and DIA (1 μg/g level), respectively, by extracting soils with TOC ≤ 2.5%. With TOC increasing to 39% recovery, values decrease up to approx. 25%. Limits of detection based on a signal-to-noise ratio of 3 and calculated on a dry-weight basis were 4 ng/g ATR and 2 ng/g DEA or DIA. Relative standard deviation of the measurement was 3.4% for ATR, 1.1% for DEA, and 3.6% for DIA. The method has been used for determination of atrazine and its degradation products in soil from maize fields.
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