Abstract
The fluorometric detection of eight previously unstudied cyclic imino acids of plant origin following automated ion-exchange fractionation is reported. cis-4-Hydroxyproline, trans-4- and cis- and trans-5-hydroxypipecolic acids, acetylaminopipecolic acid, and 2,4- trans-4,5- rans- and 2,4- trans-4,5- cis-4,5,-dihydroxypipecolic acids are resolved with a pH 3.1 buffer. Pipecolic acid is subsequently eluted by increasing the pH to 3.5. Detection involves a post-column oxidation of the secondary amines by chloramine-T prior to the introduction of the fluorogenic agent o-phthalaldehyde. The characteristic optima each compound shows with respect to the pH of the chloramine-T solution, the concentration of chloramine-T, and the temperature at which the oxidation step is carried out are reported. The method establishes the usefulness of o-phthalaldehyde for the detection of non-protein amino acids in plant material.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
