Abstract
Abstract The chemical principles used for the determination of thiamine in pharmaceutical preparations by the official USP and AOAC methods were adapted to an automated flowthrough analyzer. The system could analyze 20 samples (including blanks) per hour. Thiamine extracts from 12 different multivitamin preparations were analyzed by one analyst on 5 different days by the automated method and the AOAC method. Both methods yielded comparable values for 10 of the preparations. The thiamine content of 2 preparations containing liver extract was found to be about 9% higher by the automated method than by the AOAC method. Further analysis of other samples of these 2 preparations containing liver extract showed that chromatography on Decalso did not change the results obtained by the automated method, but raised the results obtained by the manual method to a level comparable to the automated method. The AOAC method showed a tendency for a slightly higher reproducibility of assays.
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