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Abstract
The substrate concentration is usually the most important process variable for automatic control of cultivation and for product formation. Glucose is the most popular substrate. An on-line technique for glucose analysis is described which is based on the p-HBAH method. The influence of medium components and metabolites was determined. Their influence on the measurement can be taken into account based on the calibration curves presented. This method attains the sensitivity of enzymatic glucose analysis and is less expensive and easier to handle.
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