Abstract
BackgroundIn various ICU conditions, measurement of diminished expression of human leukocyte antigen-DR on circulating monocytes (mHLA-DR) by flow cytometry appears to be a reliable marker of acquired immunosuppression. Low mHLA-DR is associated with an increased risk of nosocomial infections and mortality. Nevertheless, its use remains somewhat limited and has not been adopted in common medical practice. The main drawback of mHLA-DR measurement is likely related to the use of flow cytometry that is not accessible everywhere on a 24/7 basis. Recently, the Accellix system, a fully automated table top cytometer, was developed for use at bedside or emergency labs.MethodsThe objective was to assess the performance of the Accellix (beta site evaluation including repeatability and method comparison with reference protocol) for the measurement of mHLA-DR expression.ResultsAccellix repeatability at low and high expression levels of mHLA-DR was < 10% (i.e., within the range of acceptability for clinical flow cytometry). In comparison study including 139 blood samples (67 septic shock patients and 17 healthy volunteers), Pearson’s correlation parameters (r2) ranged from 0.71 to 0.97 (p < 0.001). Intra-class correlation coefficient was 0.92.ConclusionsThis fully automated table top cytometer appears to be a suitable tool for ICU patient monitoring and on-going clinical trials as there is no sample preparation and no need for specific skills in flow cytometry. Upon validation in a larger cohort study to reinforce reliability, Accellix could represent a major step to make flow cytometry accessible to clinicians by placing the instrument inside intensive care units or emergency laboratories.
Highlights
In various intensive care units (ICU) conditions, measurement of diminished expression of human leukocyte antigen-DR on circulating monocytes by flow cytometry appears to be a reliable marker of acquired immunosuppression [3]
Low Human leukocyte antigen-DR on monocytes (mHLA-DR) is associated with an increased risk of nosocomial infections and mortality [4]
Study population The study group consisted of 67 septic shock patients
Summary
In various ICU conditions, measurement of diminished expression of human leukocyte antigen-DR on circulating monocytes (mHLA-DR) by flow cytometry appears to be a reliable marker of acquired immunosuppression. The Accellix system, a fully automated table top cytometer, was developed for use at bedside or emergency labs. In various ICU conditions (e.g., trauma, burns, sepsis), measurement of diminished expression of human leukocyte antigen-DR on circulating monocytes (mHLA-DR) by flow cytometry appears to be a reliable marker of acquired immunosuppression [3]. Low mHLA-DR is associated with an increased risk of nosocomial infections and mortality [4]. The Accellix system, a fully automated table top cytometer requiring only introduction of the patient sample into a cartridge, was developed for use at the bedside or in emergency labs. We mainly conducted a comparison study between Accellix values and those obtained with the standardized protocol [7]
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