Abstract
4-Hydroxyproline released during collagen breakdown is not reutilised and its excretion in urine therefore reflects collagen breakdown [l]. Excretion of hydroxyproline is increased during accelerated collagen turnover such as growth [2,3], bone diseases [1,4] and tumors [5,6]. Almost all endogenous 4-hydroxyproline in man is confined to collagen, where it constitutes about 14% of the total amino acid content [7]. Quantitation of urinary 4-hydroxyproline is therefore helpful in the diagnosis and management of a variety of diseases [1,7]. Over 90% of the hydroxyproline excreted in urine is peptide bound [l]. Q uantitation of total urinary hydroxyproline therefore involves acid hydrolysis of the hydroxyproline-containing peptides. We have previously described a HPLC method for analysis of hydroxyproline in urine [8]. In this study we have modified the HPLC method to improve the accuracy of hydroxyproline measurement. The conventional spectrophotometric method by Ehrlich’s reaction after chloramine T oxidation to pyrrole [9] was also adapted to a Cobas Bio Centrifugal Analyzer for quick and automated analysis of large number of specimens.
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