Abstract
Fish silage is an autolytic product of fish or fish by-products but the autolysis does not proceed to completion. Autolysis-resistant sediment in fish silage was investigated to determine its composition, nature and reasons for insolubility. The washed and defatted sediment had poor solubility in KCl, mercaptoethanol and urea, but was largely soluble in SDS and alkali. Thawing the frozen, washed sediment separated it further into a non-sticky sediment and aqueous phase. Roughly half of the dry matter and nitrogen present in the unwashed sediment could be solubilized by simply washing with water, and most of the remaining insoluble portion dissolved in NaOH. Alkali solubilized sediment (AKSS) could be fractionated into a high MW and a low MW fraction by gel filtration on Sephadex G-50. These fractions correspond, respectively, to the precipitate and supernate of the AKSS at pH 4. SDS-PAGE of the precipitate (high MW fraction) revealed it to be a macromolecular aggregate held together by non-polar, ionic (electrostatic) and other forces.
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