Abstract
Autolysis of 21 strains of propionibacteria was studied in buffer systems. Optimal conditions for bacterial autolysis were determined. Maximal autolysis varied between 20 to 80% in potassium phosphate buffer (50mM; pH 7.0). Autolysis was maximal in the exponential growth phase at 30 to 40°C and at an ionic strength of .3. One pH optimum was observed at pH 7.2. Lysis was stimulated by Na+, K+, and NH4+ and was inhibited by different amounts of Cu2+, Zn2+, Mg2+, and Co2+. Leakage of Pro iminopeptidase followed the degree of autolysis, which verifies an intracellular location of the enzyme. Propionibacteria seemed to contain a glycosidase and an N-acetylmuramyl-L-alanine amidase or an endopeptidase.
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