Abstract
Autolysis of 21 strains of propionibacteria was studied in buffer systems. Optimal conditions for bacterial autolysis were determined. Maximal autolysis varied between 20 to 80% in potassium phosphate buffer (50mM; pH 7.0). Autolysis was maximal in the exponential growth phase at 30 to 40°C and at an ionic strength of .3. One pH optimum was observed at pH 7.2. Lysis was stimulated by Na+, K+, and NH4+ and was inhibited by different amounts of Cu2+, Zn2+, Mg2+, and Co2+. Leakage of Pro iminopeptidase followed the degree of autolysis, which verifies an intracellular location of the enzyme. Propionibacteria seemed to contain a glycosidase and an N-acetylmuramyl-L-alanine amidase or an endopeptidase.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.