Abstract
The process of autolysis in batch cultures of an industrial strain of Penicillium chrysogenum was investigated at a range of stirrer speeds. Autolysis in the cultures was monitored by conventional analysis (biomass decline, NH 4 + release), direct measurement of autolysing regions (image analysis), and enzyme activity assays (proteases and β-glucanases). Image analysis measurements provided a sensitive indicator of the onset and progress of autolysis in the culture. Autolysis could be brought about by C or n limitation. Culture history could have a marked effect on the process. Autolysis resulted in degradation of penicillin V and problems with broth filterability. It was closely associated with increased intracellular proteolytic and β-1-3-glucanolytic activities. Although stirrer speed affected both growth and penicillin V production, final mean main hyphal lengths were similar in autolysing cultures. This indicated a possible role of an intrinsic characteristic in fragmentation.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
