Abstract
ABSTRACT: This study evaluated the action of autologous platelet-rich plasma (PRP) on cutaneous wounds, containing skin autografts, in the gluteal region of horses. Seven healthy horses were used. Two 6 x 6cm cutaneous wounds were produced on each side of the gluteal region. Eight days after wound induction, grafts were performed with skin fragments harvested from the neck, as well as the application of PRP, prepared by double-centrifugation protocol. Wounds with autografts on the left side received PRP (group T), and those with autografts on the right side did not receive treatment (group C). Macroscopic and microscopic evaluations were performed, considering the integration of autografts and retraction of wound edges, as well as neovascularization, inflammatory infiltrate, young fibroblasts, collagenization, reepithelization and autografts integration. There was no difference between the groups (P > 0.05) in relation to most macroscopic and microscopic variables. However, neovascularization was significantly greater (p = 0.0191) in group T, on the 14th day after grafting. It is concluded that PRP favors the process of skin repair with autografts in horses, since it increases the neovascularization in the initial phase of wound healing. Furthermore, the PRP seems to positively influence the integration of the skin autografts and the retraction of the wound edges.
Highlights
The frequency of cutaneous injuries in horses is very high and leads to expensive treatments
Together with the fact that all the animals treated in this study showed 100% integration of autografts, reinforce the idea of the beneficial effect of platelets in the healing process of cutaneous wounds treated with autografts and platelet-rich plasma (PRP)
The microscopic variables related to young fibroblasts, re-epithelialization, inflammatory infiltrate, collagenization, and integration of autografts did not present differences between treated and control groups, which could be explained by the lower platelet concentration than that suggested by VENDRAMIN et al (2009)
Summary
The frequency of cutaneous injuries in horses is very high and leads to expensive treatments This species shows slow and difficult healing, especially in wounds located in distal limbs. This can be attributed to several factors (WILMINK et al, 1999; THEORET et al, 2001; SCHWARTZ et al, 2005; COCHRANE et al, 2017; LEPAULT et al, 2017), which leads to the development of chronic wounds (BERTONE, 1989) and, to damages to animal health. Platelet-rich plasma (PRP) is a fraction of autologous blood plasma, with a higher platelet concentration than in whole blood (MARX, 2001) It is an autogenous product with a low risk of transmission of infectious diseases (PIERCE et al, 1991) and great potential for the integration of various types of grafts (VENDRAMIN et al, 2009). The growth factors are associated with the stimulation to inflammatory response, angiogenesis, fibroblast proliferationand, increased collagen synthesis (MARX, 2004)
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