Abstract
Bovine γ/δ T cells proliferate in response to stimulation with γ-irradiated autologous monocytes in the autologous mixed leukocyte reaction (AMLR). Flow cytometric analyses indicated that the proliferating cells included three major subpopulations of bovine γ/δ T cells, distinguished by the differential expression of the γ/δ T cell receptor epitopes N6 and N7. Interleukin-2 and acid-labile interferon were produced in amlr cultures but the cultured cells did not lyse any of a large variety of target cells, including monocytes, allogeneic lymphoblasts, transformed bovine B cells (BL3), bovine fibroblast and the natural killer cell targets D17 and K562, even in the presence of lectins or after co-stimulation in the amlr with antibodies to WC1, the γ/δ T cell lineage-specific cell-surface differentiation antigen. Ex vivo γ/δ T cells did not display lymphokine-activated killing whereas populations of peripheral blood mononuclear cells containing α/β T cells did.
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