Autologous Blood Doping Induced Changes in Red Blood Cell Rheologic Parameters, RBC Age Distribution, and Performance.

Abstract

Simple SummaryAutologous blood doping (ABD) refers to the artificial increase in circulating red blood cell (RBC) mass by sampling, storage, and transfusion of one’s own blood. It is assumed that some athletes apply this prohibited technique to improve oxygen transport capacity and thus exercise performance. The primary aim of this study was to test whether RBC rheological and associated parameters significantly change due to ABD with the consideration of whether this type of measurement might be suitable for detecting ABD. Further, it was assessed whether those changes are translated into indices of endurance performance. Eight males underwent an ABD protocol combined with several blood parameter measurements and two exercise tests (pre and post transfusion). Results of this investigation suggest a change in the distribution of age-related RBC sub-populations and altered deformability of total RBC as well as of the respective sub-populations. Further, the identified changes in RBC also appear to improve sports performance. In conclusion, these data demonstrate significant changes in hematological and hemorheological parameters, which could be of interest in the context of new methods for ABD detection. However, additional research is needed with larger and more diverse study groups to widen the knowledge gained by this study.Autologous blood doping (ABD) refers to the transfusion of one’s own blood after it has been stored. Although its application is prohibited in sports, it is assumed that ABD is applied by a variety of athletes because of its benefits on exercise performance and the fact that it is not detectable so far. Therefore, this study aims at identifying changes in hematological and hemorheological parameters during the whole course of ABD procedure and to relate those changes to exercise performance. Eight healthy men conducted a 31-week ABD protocol including two blood donations and the transfusion of their own stored RBC volume corresponding to 7.7% of total blood volume. Longitudinal blood and rheological parameter measurements and analyses of RBC membrane proteins and electrolyte levels were performed. Thereby, responses of RBC sub-populations—young to old RBC—were detected. Finally, exercise tests were carried out before and after transfusion. Results indicate a higher percentage of young RBC, altered RBC deformability and electrolyte concentration due to ABD. In contrast, RBC membrane proteins remained unaffected. Running economy improved after blood transfusion. Thus, close analysis of RBC variables related to ABD detection seems feasible but should be verified in further more-detailed studies.