Autoimmunity and thrombophilia in women treated with lymphocyte immunotherapy

Abstract

able. Knowing that T cells infiltrate human decidua, we investigated the functional communications among DSC, dendritic cells (DC) and T cells, and particularly, the role of decidualized DSC on allogeneically triggered T cells by mature DC in mixed lymphocyte reaction (MLR). Methods: Decidual stromal cells from patients undergoing elective abortionswereharvested and expanded. The cellsweredecidualized in vitrowithmedroxyprogesterone, beta-estradiol and cAMP according to a standard protocol. These DSC were co-cultured with MLR, and proliferation and expression of CD25 (high affinity -chain of IL-2 receptor) and CD45RO (effector T cell isoform) on T cells were analyzed by FACS. Results: In previous reportswe showed thatDSC induce a slight proliferation of allogeneic T cells, which was even weaker when DSC were decidualized. Now, further assessment of the effect of the decidual microenvironment on effector T cells activated in MLR showed that when in a decidualized state, DSC suppress the proliferation rate of T cells to 70%. Furthermore, in contrast to CD4, cytotoxic CD8 T cells revealed a significant reduction inCD25andCD45RO markers suggesting amilder effector phenotype compared with MLR alone. Conclusion: Decidualized DSC can restrict proliferation and modulate the effector function of reactive T cells, and thus undoubtedly synergize with other physiological mechanisms for immune suppression. This study was supported by the projects: DOO250/2008 National Science Fund, Ministry of Education and Science; FP7-REGPOT-2009-1 ReProForce and BG051PO001-3.3.06-0059 the European Social Fund.