Abstract
Evidence was found for a serum immunoglobulin G (IgG) antibody against neutrophils in a patient with neutropenia and chronic active hepatitis. The patient's serum was fractionated on a DEAE chromatographic column. Each serum fraction was incubated with homologous neutrophils from the parents and siblings, and only the IgG fraction agglutinated the neutrophils. The patient's neutrophils were isolated utilizing a nylon fiber mesh. The antibody was eluted from the sensitized neutrophils and subjected to radial immunodiffusion analysis, which revealed IgG antibody. The neutrophilic antibody was further demonstrated in the serum by fluorescein-tagged anti-IgG and on the cells by means of an antiglobulin consumption test. The serum antibody was specific for human neutrophils and did not react against animal neutrophils, in contrast to systemic lupus erythematosus in which leukoagglutinins react against both human and animal granulocytes. The appearance of leukocyte autoantibodies is not surprising in a disease entity associated with a wide variety of abnormal serologic reactions.
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