Autogamy in Paramecium polycaryum

Abstract

Mass cultures of a stock of Paramecium polycaryum maintained over a period of several years showed abundant and frequent nuclear reorganization stages resembling those of ex‐conjugant and ex‐autogamous animals of other species of Paramecium. Conjugation has never been reported for P. polycaryum, nor has it been found in these studies. Cytological examination of stained preparations revealed a process of autogamy in P. polycaryum, closely similar to that described previously for P. aurelia. As a rule, all four of the micronuclei, the typical vegetative number in P. polycaryum, engage in the first prezygotic division which is characterized by the formation of prophase crescents. Variable numbers of the eight nuclei continue with the second division. A maximum of sixteen nuclei may result. Apparently, only one of these normally completes the third prezygotic division to form the gametic nuclei, although more than one may initiate it. A fusion nucleus (synkaryon) arises in, or near, a paroral cone, thus paralleling autogamy in P. aurelia. A series of postzygotic divisions produces eight definitive nuclei, four of which become macronuclear anlagen and four remain micronuclei. The first division of the synkaryon results, possibly, in the formation of a viable nucleus and a non‐viable one, as in ex‐conjugants of P. caudatum. After the last micronuclear division, a skein evolves from the old macronucleus which has become flattened and leaf‐like. The skein rapidly segments into “sausages” which transform into spherical fragments, about thirty in number. Two cell divisions restore the normal vegetative nuclear complex.