Abstract
In this paper, we demonstrate that two-photon excitation fluorescence lifetime imaging can provide critical morphological information of the retinal pigment epithelium (RPE) cells and facilitate accurate differentiation of the endogenous fluorophores in RPE cells at subcellular level via time-resolved autofluorescence measurement. Morphological and lifetime analysis show that there are two lifetime components in the RPE cells, whose lifetimes are about 620 ps and 1.4 ns respectively. Our results also reveal the spatial distribution and components of the fluorophores in the RPE cells. Combined with a laser scanning confocal ophthalmoscope, two-photon excitation fluorescence lifetime imaging might provide a novel instrumentation for the clinical diagnosis and pathological studies of age related eye diseases.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
