Abstract
The melanoma-inducing gene of Xiphophorus fish encodes the Xmrk receptor tyrosine kinase. Using a highly specific antiserum produced against the recombinant receptor expressed with a baculovirus, it is shown that Xmrk is the most abundant phosphotyrosine protein in fish melanoma and thus highly activated in the tumors. Studies on a melanoma cell line revealed that these cells produce an activity that considerably stimulates receptor autophosphorylation. The stimulating activity induces receptor down-regulation and can be depleted from the melanoma cell supernatant by the immobilized recombinant receptor protein. The fish melanoma cells can thus be considered autocrine tumor cells providing a source for future purification and characterization of the Xmrk ligand.
Highlights
The melanoma-inducing genoef Xiphophorus fishen- family and ispredicted to bind a so far unknown growth codes theXmrk receptor tyrosine kinase
Receptor tyrosine kinases arae group of transmembrane cell receptor expressed with a baculovirus, iist shown that surface receptorsw, hich mediatetheirsignaling function
Xmrk is the most abundant phosphotyrosine protein in through the activation of an intracellular tyrosine kinase dofish melanoma and highly activated in the tumorsm. ain (Ullrich and Schlessinger, 1990; Schlessinger and Ullrich, Studieson a melanoma celline revealed that these cells1992)
Summary
The melanoma-inducing genoef Xiphophorus fishen- family and ispredicted to bind a so far unknown growth codes theXmrk receptor tyrosine kinase. Immunization of Rabbits-Rabbits were immunized with the recombinant Xmrk protein produced in MNPVmrk-infected Sf9 cells.
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