Abstract
A simple continuous flow autoanalyzer for the on-line fractionation of the polyphenols content in wines is proposed. The target compounds are isolated from the matrix by solid-phase extraction on an RP-C 18 sorbent column, using selective solvents for the sequential elution of each polyphenol family. Moreover, evaporative light scattering detection (ELSD) is used for the first time for the on-line monitorization of the three polyphenol fractions present in the wine samples. Thus, a single sample injection is required to determine the global concentration of the three selected polyphenol fractions and the whole analysis is completed within a few minutes. Three calibration graphs were constructed for quantitative analysis of the global compounds concentration in every fraction and covered the range 5–300 mg l −1 (expressed as gallic acid). Average repeatability, expressed as relative standard deviation, was 4%. The proposed autonalyzer was applied to the analysis of a variety of commercial wine samples. The results obtained were compared with those provided by the Folin–Ciocalteau method, being similar in all instances.
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