Abstract
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Highlights
“To reconstruct the phylogeny of terrestrial isopods, we generated transcriptome data for 19 species available in our laboratory and used the transcriptomes of 5 other relevant crustaceans that were publicly available (Supplementary Table S1)
Out of 22 isopod species, we found 76–88 markers in all species but Helleria brevicornis (67 markers) and Asellus aquaticus (66 markers)
Two subspecies for which the heterogametic system is known were not included in our RNA-seq experiment: Porcellio dilatatus dilatatus (XY/XX)42 and Porcellio dilatatus petiti (ZZ/ZW)43
Summary
“To reconstruct the phylogeny of terrestrial isopods, we generated transcriptome data for 19 species available in our laboratory and used the transcriptomes of 5 other relevant crustaceans that were publicly available (Supplementary Table S1). Among the 35 markers that independently produced a topology identical to the one retrieved with the combined alignment, we selected the 10 longest ones (5 nuclear and 5 mitochondrial) and Sanger-sequenced them in the two P. dilatatus subspecies.
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