AUTHENTICATION OF TURMERIC USING PROTON-NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY AND MULTIVARIATE ANALYSIS

Anjar Windarsih,Abdul Rohman,Respati Tri Swasono

doi:10.22159/ijap.2018v10i6.29014

Abstract

Objective: The objective of this study was to apply 1H-NMR spectroscopy-based metabolite fingerprinting in combination with multivariate analysis for authentication of turmeric (Curcuma longa) from C. heyneana and C. manga.Methods: Partial least square-discriminant analysis (PLS-DA) and orthogonal projections to latent structures-discriminant analysis (OPLS-DA) were used for differentiation of authentic and adulterated C. longa with C. manga and C. heyneana. The variables used were peaks with certain chemical shifts at optimized 1H-NMR spectra of authentic and adulterated C. longa.Results: All of the authentic C. longa samples were clearly separated from the adulterated ones. The multivariate calibration of partial least square (PLS) was successfully applied to predict of adulterants in C. longa. The lower RMSEC (root mean square error of calibration) values, 0.94% for adulterated C. longa with C. heyneana and 1.37% for adulterated C. longa with C. manga, and the lower RMSEP (root mean square error of prediction) values, 0.83% for adulterated C. longa with C. heyneana and 1.34% for adulterated C. longa with C. manga indicated the good of accuracy and precision of the calibration models.Conclusion: The combination of 1H-NMR spectroscopy and chemometrics of multivariate analysis PLS-DA, OPLS-DA, and PLS proves an adequate technique for authentication of turmeric.

Highlights

Curcuma longa is one of the herbaceous perennial used for a long time in the field of foodstuff, cosmetics, and traditional medicine [1], with several pharmacological activities of antioxidant [2], antiinflammatory [3], antibacterial, hepatoprotective, cardioprotective [4, 5], maintain gastrointestinal tract, and chemoresistance activities [6] due to curcuminoids compound
1H-NMR spectroscopy is capable of measuring spectra of C. longa, C. heyneana, and C. manga
The spectra of adulterated C. longa with 25% of C. heyneana and 25% of C. manga showed a similar pattern with the spectra of authentic C. longa

Summary

Introduction

Curcuma longa is one of the herbaceous perennial used for a long time in the field of foodstuff, cosmetics, and traditional medicine [1], with several pharmacological activities of antioxidant [2], antiinflammatory [3], antibacterial, hepatoprotective, cardioprotective [4, 5], maintain gastrointestinal tract, and chemoresistance activities [6] due to curcuminoids compound (curcumin, demethoxycurcumin, and bisdemethoxycurcumin). The nondestructive and reliable methods including ultraviolet-visible spectroscopy, Fourier Transform Raman spectroscopy, near infrared, attenuated total reflectance (mid-infrared) spectroscopy [15,16,17], and proton nuclear magnetic resonance (1H-NMR) spectroscopy have been applied for authentication in foods and medicinal plants [18]. The latter, 1H-NMR spectroscopy offered great advantages in metabolomics study for authentication of medicinal plants [19, 20] especially in combination with chemometrics of partial least square-discriminant analysis (PLS-DA) and orthogonal projections to latent structures-discriminant analysis (OPLS-DA). A combination of 1H-NMR spectroscopy and multivariate analysis of pattern recognition and regression was carried out for the authentication of C. longa adulterated with C. heyneana and C. manga

Methods

Results

Conclusion