Abstract
Extraction of dye from dry fruit of Rothmannia whitfieldii was carried out using four different extraction methods. Solvent and acid extraction methods gave a colourless supernatant solution after extraction time of 45 minutes at 60°C. The alkali method gave a deep brown coloured supernatant solution while the aqueous method gave a dark coloured supernatant solution after extraction under the same conditions. From the result of the FTIR spectroscopy characterization of the coloured solutions and the dry powder of Rothmannia whitfieldii fruit, it was observed that only the alkali method extracted what can be called a dye with likely presence of tannins. The result also showed that the possible functional groups present in the supernatant solution after aqueous extraction are same with the functional groups present in the dry pulverized Rothmannia whitfieldii fruit. Hence, aqueous method did not extract any dye. Similarly, a mixture of the solution after aqueous extraction with drops of alkali solution produced a deep brown coloured solution indicating solubility of the dye component in alkali media.
Highlights
Interest in the study and use of natural dyes especially from plant materials in recent times is on the increase mostly due to environmental hazard and toxic effect of most synthetic dyes on the human skin [1] [2]
It was observed that 5 ml of the supernatant liquid from the aqueous extraction when mixed with drops of 1% NaOH solution changed colour from black to deep brown
This perhaps indicates that the natural dye molecule in dry Rothmannia whitfieldii fruit does not dissolve in water but dissolves in alkali solution
Summary
Interest in the study and use of natural dyes especially from plant materials in recent times is on the increase mostly due to environmental hazard and toxic effect of most synthetic dyes on the human skin [1] [2]. Of all the known sources of natural dye, plant materials are mostly used. It has been observed that different coloured extract can be achieved from a particular plant source depending on the extraction method used. A major challenge is how to ascertain the extraction method that will yield a dye and how to confirm which of the coloured extract can be considered a dye
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