Abstract
Black yeast, Aureobasidium pullulans is extracellularly produced β-(1,3), (1,6)-D-glucan (β-glucan) under certain conditions. In this study, using Glycine max cv. Kurosengoku (Kurosengoku soybeans), the production of β-glucan through fermentation of A. pullulans was evaluated, and the effects of A. pullulans cultured fluid (AP-CF) containing β-glucan made with Kurosengoku soybeans (kAP-CF) on a human monocyte derived cell line, Mono Mac 6 cells were investigated. Concentration of β-glucan in kAP-CF reached the same level as normal AP-CF. An anti-angiogenic protein, Thrombospondin-1 (THBS1) was effectively induced after the stimulation with kAP-CF for comparison with AP-CF. The THBS1 is also induced after stimulation with hot water extract of Kurosengoku soybeans (KS-E), while the combined stimulation of β-glucan with KS-E more effectively induced THBS1 than that with KS-E alone. These results suggest effects of A. pullulans-produced β-glucan on the enhancement of Kurosengoku soybean-induced THBS1 expression.
Highlights
A black yeast, Aureobasidium pullulans, extracellularly produces a β-(1, 3), (1, 6)-D-glucan (β-glucan) under certain conditions
The results indicate that the ground Kurosengoku soybeans may be substituted for and used as the nitrogen source in the fermentation of A. pullulans for β-glucan production
To assess the kAP-CF and compare it with conventional A. pullulans-cultured fluid (AP-CF) made with rice bran, a human, acute monocytic leukemia derived cell line, Mono Mac 6 cells were stimulated with kAP-CF, and the expression of mRNAs was evaluated using primer arrays
Summary
A black yeast, Aureobasidium pullulans, extracellularly produces a β-(1, 3), (1, 6)-D-glucan (β-glucan) under certain conditions. The A. pullulans-produced β-glucan is known to be a dietary fiber and an immune stimulator, and it is believed to provide beneficial effects to health through its function as a dietary fiber and its immuno-stimulating activity. Activation of type-1 immunity is involved in the prevention of tumor[23] formation and allergic diseases[24] These effects resemble the effects of β-glucan, and may be anticipated to be enhanced by the combined administration of A. pullulans together with Kurorengoku soybeans. The THBS1 induction activity of KS-E is mainly dependent on soy isoflavones, and the THBS1 induction activities of daidzein and genistein are higher than that of glycitein These results suggest that β-glucan is effective in enhancing the effects of soy isoflavones on the cells
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