Augmented mechanical response of muscular thin-fiber receptors in aged rats recorded in vitro

Abstract

Musculoskeletal pain deteriorates quality of life by disrupting daily activities and is a considerable economic burden to many countries because of the large number of patients. Little is known about the peripheral neural mechanisms of muscular nociception in the aged, although structural and functional changes in the muscle are apparent as a function of age. The aim of the present study was to investigate the activities of aged muscle nociceptors systematically to mechanical, chemical and thermal stimuli, and to compare with the data from young animals. Activities of single C-fibers were recorded from in vitro preparations of extensor digitorum longus muscle–nerve excised from hind legs of aged rats (125–133 weeks). Mechanical threshold measured by a ramp mechanical stimulus in the aged muscle (median; 45.2 mN (IQR; 38.1–59.1 mN), n = 29) was significantly lower than that in the younger muscle (median; 65.4 mN (IQR; 46.6–122.0 mN), n = 33, p < 0.01, Mann–Whitney U-test) reported in our previous study ( Taguchi et al., 2005). In addition, the magnitude of the mechanical response during the first 5 s of the 10 s stimulus was significantly greater in the aged muscle (11.0 spikes (IQR; 6.5–20.5 spikes)) than in the young (7.0 spikes (IQR; 4.0–11.5 spikes), p < 0.05, Mann–Whitney U-test). In contrast, the numbers of discharges induced by chemical (pH 5.5, ATP and bradykinin) and thermal (cold and heat) stimuli were not different with the different ages. These results showed an augmented mechanical response in muscle C-afferents in the aged rats.