Augmentation of porcine spontaneous cell-mediated cytotoxicity in vitro.

Abstract

Porcine spontaneous cell-mediated cytotoxicity (SCC) was recognized in spleen and peripheral blood lymphocytes, and the cytotoxicity of spleen mononuclear lymphocytes were higher than that of peripheral blood mononuclear cells against K-562 human myeloid cell. Scc was augmented spontaneously when incubated lymphocytes at 37°C with 10% fetal calf serum containing medium or serum free media, however, their augmentation of SCC was reduced by autologous and homologous serum. The maximum augmentation of SCC by incubation was achieved at 40 hr, the kinetics was correlated with the DNA synthesis of whole mononuclear lymphocytes. When adherent cells (ADC) were removed from whole peripheral mononuclear cells (WPBL) or whole spleen mononuclear lymphocytes (WSL), SCC was not augmented by incubation at 37°C. Addition of cultured fluid from WPBL or WSL on non-adherent lymhocyte (NAC) culture, augmentation of SCC by incubation was also recognized. IL-1 and IL-2 like activity was detected in these cultured fluids from WPBL and WSL. Porcine SCC was also boosted in vitro by incubation with IFN-γ inducer OK-432. This augmentation was not dependent on ADC. These results suggested that spontaneous augmentation of porcine SCC is dependent on the formation of IL-1 by ADC and IL-2 by NAC. Also IFN-γ may play an important role of SCC augmentation in vitro.